In 2010, a significant scientific milestone was achieved for tree fruit crops: publication of the primary draft whole genome sequence (WGS) for apple (Malus domestica).
This WGS, v1.0, was precious because the preliminary reference for sequence info, wonderful mapping, gene discovery, variant discovery, and device growth. A new, top quality apple WGS, GDDH13 v1.1, was launched in 2017 and now serves because the reference genome for apple.
Over the previous decade, these apple WGSs have had an unlimited affect on our understanding of apple organic functioning, trait physiology and inheritance, resulting in sensible functions for enhancing this extremely valued crop. Causal gene identities for phenotypes of elementary and sensible curiosity can immediately be found far more quickly.
Genome-wide polymorphisms at excessive genetic decision are screened effectively over lots of to 1000’s of people with new insights into genetic relationships and pedigrees. High-density genetic maps are constructed effectively and quantitative trait loci for precious traits are readily related to positional candidate genes and/or transformed into diagnostic assessments for breeders.
We perceive the species, geographical, and genomic origins of domesticated apple extra exactly, in addition to its relationship to wild kin. The WGS has turbo-charged software of those classical analysis steps to crop enchancment and drives modern strategies to attain extra sturdy, environmentally sound, productive, and consumer-desirable apple manufacturing.
This overview consists of examples of fundamental and sensible breakthroughs and challenges in utilizing the apple WGSs. Recommendations for “what’s subsequent” deal with crucial upgrades to the genome sequence information pool, in addition to to be used of the information, to achieve new frontiers in genomics-based scientific understanding of apple.
Field Evaluation of a New Sequential Sampling Technique for Determining Apple Scab “Risk”.
Most fungicide sprays utilized to apple orchards within the New England states are focused on the administration of apple scab. Researchers have developed motion thresholds that help in decision-making on whether or not early spring fungicide functions might be eradicated with no vital improve within the incidence of fruit scab at harvest. To facilitate grower adoption of those thresholds, a simplified, sequential sampling method in autumn to find out the “scab danger” of an orchard for the next spring was proposed within the scientific literature.
However, this system had not been evaluated within the subject. In autumn 1999, 2000, and 2001, orchards have been evaluated utilizing the new sequential sampling method to find out scab danger. Risk rankings have been in contrast with these obtained by the unique, nonsequential process in every orchard.
Data additionally have been examined utilizing a simulation sequential sampling laptop program to find out whether or not or not danger rankings would change if completely different bushes or shoots have been used. In two of the assessed orchards, “delayed-spray” experiments involving two therapies (a delayed-spray and full-spray remedy) have been performed in 2000 and 2001.
 maxi blotter 20x20 cm |
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| EVS1300-BLOT | Consort | ea | EUR 1113 |
 mini blotter 10x10 cm |
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| EVS3100-BLOT | Consort | ea | EUR 986 |
maxi blotter 20x20 cm |
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| EVS3300-BLOT | Consort | ea | EUR 1366 |
 Western Blot Kit |
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| 20-abx098123 | Abbexa |
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 (A/northern shoveler/California/HKWF115/07) [His]) IAV H6N1 (aa 17-529) (A/northern shoveler/California/HKWF115/07) [His] |
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| DAG1686 | Creative Diagnostics | 50 ug | EUR 1681 |
 Western Blot Enhancing Kit |
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| K3172120 | Biochain | 1200 cm2 | EUR 143 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Western Blot Enhancing Kit |
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| K3172250 | Biochain | 2500 cm2 | EUR 188 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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 BlotBot Mini Blot Tray |
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| TRAY-2MINI-2 | Next Advance | 2pack | EUR 204 |
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Description: Replacement trays for the BlotBot allows you to run 2 Mini sized blots simultaneaously. Pack of 2 trays. |
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 BlotBot Midi Blot Tray |
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| TRAY-MIDI-2 | Next Advance | 2pack | EUR 202 |
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Description: Replacement tray for the BlotBot allows you to run 1 Midi sized blot. Pack of 2 trays. |
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 TMB Substrate, for Western Blot |
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| F100029 | Cygnus Technologies | 1000 ml | EUR 827 |
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Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur. |
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TMB Substrate, for Western Blot |
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| F129-100 | Cygnus Technologies | 100 ml | EUR 249 |
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Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur. |
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 mini blot insert 10x10 cm |
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| EVS1100-BLOTINSERT | Consort | ea | EUR 411 |
 mini blot casette 10x10 cm |
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| EVS1100-CASETTE | Consort | ea | EUR 65 |
 miniwide blot insert 20x10 cm |
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| EVS1200-BLOTINSERT | Consort | ea | EUR 470 |
 maxi blot insert 20x20 cm |
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| EVS1300-BLOTINSERT | Consort | ea | EUR 589 |
 maxi blot casette 20x20 cm |
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| EVS1300-CASETTE | Consort | ea | EUR 82 |
mini blot casette 10x10 cm |
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| EVS3100-CASETTE | Consort | ea | EUR 132 |
maxi blot casette 20x20 cm |
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| EVS3300-CASETTE | Consort | ea | EUR 158 |
 Interferon alpha Western Blot Antibody |
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| 20-abx137014 | Abbexa |
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 WSE-4055 Q Blot kit |
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| 2322445 | Atto | 2unit | EUR 385 |
 AgileBlotter? Automated Western Blot Processor |
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| ACT-ABH-40 | ACTGene | EUR 16939 | |
 Hypersensitive Western Blot Chemiluminescent Substrate |
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| CR0001-10 | BosterBio | 20mL (sufficient reagents for 4000 cm2 of membrane) | EUR 167 |
Hypersensitive Western Blot Chemiluminescent Substrate |
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| CR0001-25 | BosterBio | 50mL (sufficient reagents for 10000 cm2 of membrane) | EUR 314 |
Hypersensitive Western Blot Chemiluminescent Substrate |
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| CR0001-5 | BosterBio | 10mL (sufficient reagents for 2000 cm2 of membrane) | EUR 116 |
) PDCD4 Western Blot kit (AWBK09045) |
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| AWBK09045 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) ETS1 Western Blot kit (AWBK100604) |
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| AWBK100604 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) ERG Western Blot kit (AWBK100625) |
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| AWBK100625 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) GABRP Western Blot kit (AWBK13034) |
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| AWBK13034 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) HTR1A Western Blot kit (AWBK13041) |
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| AWBK13041 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) RAB14 Western Blot kit (AWBK13107) |
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| AWBK13107 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) TNRC4 Western Blot kit (AWBK31525) |
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| AWBK31525 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) ESRRG Western Blot kit (AWBK31655) |
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| AWBK31655 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) GLI2 Western Blot kit (AWBK31885) |
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| AWBK31885 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) PAX4 Western Blot kit (AWBK32064) |
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| AWBK32064 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) AHR Western Blot kit (AWBK32243) |
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| AWBK32243 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) GLI1 Western Blot kit (AWBK32368) |
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| AWBK32368 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) PAX7 Western Blot kit (AWBK32393) |
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| AWBK32393 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) LHX6 Western Blot kit (AWBK32553) |
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| AWBK32553 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) HIPK2 Western Blot kit (AWBK32586) |
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| AWBK32586 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) PAX7 Western Blot kit (AWBK32742) |
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| AWBK32742 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) ZFP67 Western Blot kit (AWBK32749) |
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| AWBK32749 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) OLIG2 Western Blot kit (AWBK32753) |
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| AWBK32753 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) ENG Western Blot kit (AWBK33068) |
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| AWBK33068 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) SOX5 Western Blot kit (AWBK33323) |
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| AWBK33323 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) SOX10 Western Blot kit (AWBK33326) |
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| AWBK33326 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) RPS16 Western Blot kit (AWBK33535) |
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| AWBK33535 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) ATP7A Western Blot kit (AWBK33797) |
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| AWBK33797 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) A1BG Western Blot kit (AWBK33810) |
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| AWBK33810 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) NPM1 Western Blot kit (AWBK34094) |
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| AWBK34094 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) LIG4 Western Blot kit (AWBK34122) |
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| AWBK34122 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) RNF2 Western Blot kit (AWBK34290) |
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| AWBK34290 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) ZFP36 Western Blot kit (AWBK34385) |
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| AWBK34385 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) ARIH2 Western Blot kit (AWBK34418) |
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| AWBK34418 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) KEAP1 Western Blot kit (AWBK34728) |
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| AWBK34728 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) CLIC1 Western Blot kit (AWBK35045) |
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| AWBK35045 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) KCNN2 Western Blot kit (AWBK35094) |
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| AWBK35094 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) CLCN3 Western Blot kit (AWBK35504) |
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| AWBK35504 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) P2RX7 Western Blot kit (AWBK35518) |
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| AWBK35518 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) TRPM3 Western Blot kit (AWBK35609) |
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| AWBK35609 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) PITX2 Western Blot kit (AWBK35634) |
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| AWBK35634 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) ILF2 Western Blot kit (AWBK35731) |
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| AWBK35731 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) ONECUT2 Western Blot kit (AWBK35750) |
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| AWBK35750 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) ZFP42 Western Blot kit (AWBK36244) |
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| AWBK36244 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) ANXA1 Western Blot kit (AWBK36568) |
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| AWBK36568 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) SPP1 Western Blot kit (AWBK36677) |
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| AWBK36677 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) RUNX2 Western Blot kit (AWBK36678) |
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| AWBK36678 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) ZBP1 Western Blot kit (AWBK36711) |
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| AWBK36711 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) Bapx1 Western Blot kit (AWBK36826) |
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| AWBK36826 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) G3BP Western Blot kit (AWBK37713) |
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| AWBK37713 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) TWIST1 Western Blot kit (AWBK37997) |
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| AWBK37997 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) ID4 Western Blot kit (AWBK38058) |
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| AWBK38058 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) SATB1 Western Blot kit (AWBK38208) |
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| AWBK38208 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) EED Western Blot kit (AWBK38384) |
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| AWBK38384 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) TARDBP Western Blot kit (AWBK38941) |
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| AWBK38941 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) TARDBP Western Blot kit (AWBK38942) |
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| AWBK38942 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) BRD7 Western Blot kit (AWBK39018) |
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| AWBK39018 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) JMJD3 Western Blot kit (AWBK40102) |
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| AWBK40102 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) SNRP70 Western Blot kit (AWBK40439) |
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| AWBK40439 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) SSB Western Blot kit (AWBK40462) |
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| AWBK40462 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) STAU1 Western Blot kit (AWBK40537) |
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| AWBK40537 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) PCBP1 Western Blot kit (AWBK40630) |
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| AWBK40630 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) HNRPLL Western Blot kit (AWBK41102) |
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| AWBK41102 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) MSI2 Western Blot kit (AWBK41112) |
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| AWBK41112 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) HNRPA3 Western Blot kit (AWBK41195) |
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| AWBK41195 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) WNT2B Western Blot kit (AWBK41254) |
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| AWBK41254 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) GNB1L Western Blot kit (AWBK41328) |
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| AWBK41328 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) SLC12A1 Western Blot kit (AWBK41388) |
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| AWBK41388 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) TPM1 Western Blot kit (AWBK41392) |
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| AWBK41392 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) CEACAM6 Western Blot kit (AWBK41504) |
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| AWBK41504 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) SLC22A1 Western Blot kit (AWBK41516) |
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| AWBK41516 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) STK11 Western Blot kit (AWBK41743) |
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| AWBK41743 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) OTC Western Blot kit (AWBK41766) |
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| AWBK41766 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) TSHR Western Blot kit (AWBK41870) |
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| AWBK41870 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) RHOBTB1 Western Blot kit (AWBK41883) |
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| AWBK41883 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) KRT13 Western Blot kit (AWBK42031) |
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| AWBK42031 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) IDH3A Western Blot kit (AWBK42237) |
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| AWBK42237 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) SLC1A5 Western Blot kit (AWBK42247) |
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| AWBK42247 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) PBEF1 Western Blot kit (AWBK42255) |
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| AWBK42255 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) FST Western Blot kit (AWBK42287) |
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| AWBK42287 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) SLC22A7 Western Blot kit (AWBK42306) |
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| AWBK42306 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) ASPN Western Blot kit (AWBK42487) |
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| AWBK42487 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) RSAD2 Western Blot kit (AWBK42696) |
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| AWBK42696 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) PANX2 Western Blot kit (AWBK42778) |
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| AWBK42778 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) PANX1 Western Blot kit (AWBK42783) |
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| AWBK42783 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) TGFBI Western Blot kit (AWBK44268) |
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| AWBK44268 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) PTPN2 Western Blot kit (AWBK45362) |
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| AWBK45362 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) RORA Western Blot kit (AWBK45608) |
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| AWBK45608 | Aviva Systems Biology | 10 reactions | EUR 647 |
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Description:
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) Chemiluminescent Western Blot Kit (OKRA00001) |
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| OKRA00001 | Aviva Systems Biology | 1 kit | EUR 570 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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) Chemiluminescent Western Blot Kit (OKRA00002) |
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| OKRA00002 | Aviva Systems Biology | 1 kit | EUR 570 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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) Chemiluminescent Western Blot Kit (OKRA00003) |
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| OKRA00003 | Aviva Systems Biology | 1 kit | EUR 570 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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) Chemiluminescent Western Blot Kit (OKRA00004) |
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| OKRA00004 | Aviva Systems Biology | 1 kit | EUR 570 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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) Colorimetric Western Blot Kit (OKRA00014) |
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| OKRA00014 | Aviva Systems Biology | 1 kit | EUR 609 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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) Colorimetric Western Blot Kit (OKRA00015) |
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| OKRA00015 | Aviva Systems Biology | 1 each | EUR 609 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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) Colorimetric Western Blot Kit (OKRA00016) |
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| OKRA00016 | Aviva Systems Biology | 1 kit | EUR 609 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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) Colorimetric Western Blot Kit (OKRA00017) |
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| OKRA00017 | Aviva Systems Biology | 1 kit | EUR 609 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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 mini-wide blot casette 20x10 cm |
|||
| EVS1200-CASETTE | Consort | ea | EUR 65 |
 T-Pro Western Blot Stripping Reagent |
|||
| JB11-K002 | T-Pro Biotechnology | 500ml/BT | EUR 178 |
 Western Blot Primary Antibody Diluent Buffer |
|||
| abx090689-100ml | Abbexa | 100 ml | EUR 182 |
|
|||
) ChonBlock Western Blot Buffer 1 (PBS) |
|||
| 9069P | Chondrex | 10 ml | EUR 193.55 |
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Description: ChonBlock Western Blot Buffer 1 (PBS) |
|||
) ChonBlock Western Blot Buffer 1 (TBS) |
|||
| 9069T | Chondrex | 10 ml | EUR 193.55 |
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Description: ChonBlock Western Blot Buffer 1 (TBS) |
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Delayed-spray replicates have been to obtain no fungicide sprays till after the third major an infection interval (however earlier than the fourth) or till the pink stage of bud growth, whichever got here first; full-spray replicates acquired fungicide sprays beginning on the green-tip stage of bud growth.
The sequential sampling method offered scab-risk rankings per the unique, nonsequential process, at doubtlessly vital time financial savings. Also, following the delayed-spray technique in low-risk orchards didn’t lead to vital variations in fruit scab at harvest in contrast with initiating spraying on the green-tip phenological bud stage.